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KMID : 0604520080340010025
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Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 1 p.25 ~ p.35
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Antioxidative Activities and Antiaging Effects of Geranium nepalense Extracts
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Lee Keun-Ha
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects and inhibitory effects on elastase and tyrosinase of Geranium nepalense extracts were investigated. The free radical(1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities () of extract/fractions of Geranium nepalense were in the order: 50% ethanol extract(15.0 )). ). Reactive oxygen species (ROS) scavenging activities() of some Geranium nepalense extracts on ROS generated in system were investigated using the luminol-dependent chemiluminescense assay. The order of ROS scavenging activities were 50% ethanol extract())). Deglycosylated flavonoid fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Geranium nepalense on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Geranium nepalense extracts suppressed photohemolysis in a concentration dependent manner, particularly deglycosylated flavonoid fraction exhibited the most prominent celluar protective effect (, 676.7 min at 50 ). The inhibitory effect of aglycone fraction on tyrosinase(, 70.0 ) and elastase (, 19.9 ) was very high. Aglycone fractions obtained from the deglycosylation reaction of ethyl-acetate fraction among the Geranium nepalense extracts, showed 2 bands in TLC and 2 peaks in HPLC experiments (370 nm). Two components were identified as quercetin(composition ratio, 15.3%), kaempferol(82.8%). These results indicate that extract/fractions of Geranium nepalense can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging and other ROS, and protect cellular membranes against ROS. And component analysis of Geranium nepalense extract and inhibitory activity on elastase of the aglycone fraction could be applicable to new functional cosmetics for smoothing wrinkles.
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KEYWORD
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Geranium nepalense, flavonoid, antioxidative, elastase, cosmetics
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